Composite

Part:BBa_K4072004

Designed by: Barney Geddes   Group: iGEM21_NDSU   (2021-10-19)


Constitutive chiA

Promoter J23106_AB (CIDAR), RBS BCD12_BC (CIDAR), ORF chiA_CD (BBaK072000), and Terminator (B0015_DE) combined into vector backbone DVK_AE (CIDAR) by BsaI golden gate cloning. Confirmed by PCR and restriction digest, did not show antifungal activity vs Rhizoctinia solani in a plate fungal inhibition assay when expressed in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2035
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2076
    Illegal AgeI site found at 1398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 148
    Illegal BsaI.rc site found at 2186


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Categories
Parameters
None